The ‘Nuts & Bolts’

We are very excited to have you work with us in the Exercise Physiology Research Laboratory. Please acquaint yourself with this manual. Most, if not all, of your questions should be addressed. In regards to successful completion of this lab experience, the burden of responsibility will be on you, the student, and not the faculty. So please ask questions beforehand to prevent future, unforeseen problems.

Introduction and Orientation

Both the SRP 99 and 199 lab experience courses are designed to provide you with a wide scope of laboratory and practitioner skills within the exercise physiology realm. As such you will be required to fulfill the obligations outlined in the following pages as well as perform the duties required by your lab supervisor.

**If you are a student high school volunteer for the summer, or a medical/dental student intern for the summer, you will adhere to the purpose and goals of the lab experience forthcoming, however your application process won’t follow the SRP electives application but rather an application process developed by the UCLA Health System.**

In participating in an experience of this nature, it is good to remember that you are not only representing this lab and UCLA, but you are also representing yourself. Your success in this venture will depend upon the impressions you create and the abilities you display in performing assignments and tasks. This experience should be looked at as a privilege. Consequently, the student should be passionate and show a genuine interest.

Purpose of Lab Experience

To provide students an opportunity to have practical experience in a laboratory setting that is providing established professional health-fitness services commensurate with the student’s educational level, previous experience and professional goals. The laboratory experience shall augment the formal classroom and related learning experiences.

General Goals of Lab Experience

The goals of your lab experience include, but are not limited to, the following:
1. Provide undergraduate students an opportunity to gain practical experience and appreciation of the role, duties, and responsibilities of working in a physiology-based laboratory.
2. Through involvement with applied research studies in the laboratory, students will learn to augment health-fitness assessments and prescriptions and to utilize their acquired knowledge by bridging-the-gap from classroom theory to practical, day-to-day application.
3. Provide an opportunity for the undergraduate student to establish realistic goals (based on their experiences and performance) for their own professional development.
4. Provide an opportunity for undergraduate students to have the EPRL supervisor evaluate their skills and competencies

Roles in the Lab

These are some of the expectations for student tasks in the EPRL. These may vary depending on the on-going laboratory projects.
1. Administrative duties
a. Coordinate the team’s calendar and subject scheduling
i. Schedule a subject for testing using Doodle or Google
ii. Communicate the date/time of test to the research team
iii. Designate/confirm the available personnel to attend and run the tests
b. Create subject folders and communicate with the team where important information is located
i. Study form copies
ii. De-identified subject information files
iii. Secure patient identifier binder
iv. Safety information
v. Informed Consent Forms
vi. Test protocols and instructions
vii. Troubleshooting logs and tips
viii. Task lists and checklists
c. Create standard scripts for thorough data collection and efficiency of testing
2. Equipment management
a. Understand standard operating procedures for equipment
b. Calibrate appropriate equipment at least 15 minutes before subject arrival
c. Secure equipment on subject
d. Monitor data flow for abnormalities and errors
e. Operate equipment and place time markers on data
f. Note and log any troubleshooting issues
g. Save test results and make redundant backups
3. Subject prep and fitness assessments
a. Create and compile subject information file
i. Label file with identifier number
ii. Include copies of appropriate forms (2 ICFs, subject Instruction/Compliance Checklist, Payment Form, Data Sheets and Questionnaires)
b. Review subject instruction sheet and run through list for compliance
c. Administer pre-exercise interview and have subject review and sign informed consent
i. Signatures required from test subject and from study investigator (Provide 1 copy for subject and keep 1 copy in locked ICF binder and label with identifier number)
ii. Fill out online consent if applicable and keep hard copy in binder d. Administer pre-exercise interview
e. Uses exercise testing equipment, protocol selection, established guidelines for indications, contraindications, and termination criteria for cardiopulmonary exercise testing, muscle strength, power and endurance testing, functional screening, balance testing and body composition assessments.
f. Administers progressive exercise training and other techniques designed to provide improved functional capacity, exercise tolerance and endurance, muscle strength and endurance, considering the subject’s condition and behaviors
g. Evaluates patient hemodynamic, electrocardiographic, and symptomatic response to exercise, and assesses appropriateness of responses as they relate to exercise intensity and endurance
h. Provide water and constantly make inquiries about their state of health (especially during maximal testing)
i. There may be times that travel is required to a remote testing site (e.g., Equinox gym in Century City)
j. Assists in developing and providing educational materials for patients
4. Laboratory and Equipment Maintenance
a. Run through clean-up checklist and sign name at the end of testing
b. Recharge batteries for Oxycon Mobile
c. Charge heart rate devices and Android phones
d. Plug equipment/computer back
e. Turn off calibration gas
f. Decontaminate and wipe clean equipment (masks, Polar belts, ECG wires)
g. Put away or lock-up equipment in designated spaces
h. Hang EKG leads and sampling lines to dry
i. Place trash can in hallway to be emptied
5. Quarterly calibration of equipment
a. Perform Human calibration of Oxycon Pro and Mobiles (see Appendix ‘Human Calibration Protocol’
b. Calibrate Treadmill and cycle ergometer
6. Manuscript preparation
a. Be proficient with standard Microsoft programs (Word, Excel, Powerpoint) and web browsers
b. Write research text for publication
c. Write summaries for presentations, reports, manuscripts and to summarize results of analyses in graphs, tables and figures
d. Search PubMed by topic for thorough and comprehensive summary of current research and theory

Program description

An agreement is established between an EPRL supervisor and the student, as to provide a comprehensive laboratory learning experience for those pursuing a Bachelor’s degree.

The duration of this lab experience is based on the number of credit hours in which the student is enrolled. In the case of those with Freshman or Sophomore status, you can only enroll in SRP 99 (which is worth 1-2 credit hours); thereby equating into a minimum of 1-2 weekly contact hours in the laboratory. Those students with Junior or Senior status may enroll in SRP 199 (which can be worth 1-4 credit hours); thereby equating into a minimum of 4-8 weekly contact hours in the laboratory. Lab experience hours must be non-paid.

In order to maximize student learning certain expectations and responsibilities must be assumed by the student. If the student continually violates any policy or procedure, the supervisor may terminate the student’s affiliation at any time with subsequent loss of one hour of academic credit. Although not inclusive, some of the student’s expectations to the lab are:

• Good academic standing with UCLA
• Providing assigned laboratory services in a professional manner that follow an ethical code of conduct (as outlined on UCLA’s webpage).
• Exercising good judgment concerning dress, speech, and personal habits
• Relating to colleagues and participants and with respect and dignity while adhering to their rights
• Conducting himself/herself in a manner, which reflects well upon the student, laboratory, and the University
• Working within the scope of laboratory and university policies and procedures
• Displaying interest, resourcefulness, and commitment in attacking problems and suggesting solutions
• Ability to work independently with minimal supervision and be able to proceed without specific direction to organize and execute research plan


The following procedures have been updated and approved for the Spring 2015 academic year and beyond.

Prerequisites for enrollment

The following prerequisites must be met before enrollment in the lab experiences courses:
• We prefer students pursuing a degree in Integrative Biology and Physiology or equivalent science major at UCLA
• The student must maintain at least a 3.0 G.P.A. during their lab experience


The following guidelines have been established for acceptance, enrollment and subsequent completion of the lab experience courses:
• Ideally, during the quarter prior to which the student plans to complete their lab experience, they must follow the ‘3-step Process for Potential EPRL Student Interns’ (in Forms) that was emailed by an EPRL staff member. This includes an ‘Initial EPRL Interest’ questions completed by the student and emailed back to the EPRL staff.
• After a selection process within the EPRL staff, students will be contacted via email if they wish to secure credit for a lab experience.
• The student should attempt to secure their chosen lab experience before quarter’s end and prior to the start of the next quarter in which they want to complete the experience
• Once a verbal agreement is made between student and EPRL staff, the student must print out and bring in a ‘Student EPR Lab Experience Form’ (in Appendix) to the lab to be filled out with their assigned supervisor.
• Student must go online to “myucla” and select “contract courses” and then elect “create new contract” and choose the appropriate course (SRP 99 or SRP 199). Then fill in department as “medicine” and supervisor/PI name as “COOPER, CHRISTOPHER, PULMONOLOGY”
• Continue to fill-out the contract details that you and an EPRL supervisor agreed to (a sample ‘SRP 199’ and ‘SRP 99’ is found in the Appendix of this manual) and then agree to the terms and conditions.
• An EH&S safety course is MANDATORY. If you haven't done the EH&S safety course (it is about 2-3 hours and free), you can view available classes at (fill in the following when signing up - Department: Medicine-Pulmonary, Lab Address: CHS 52-065, Lab extension: x42156, Lab PI: Christopher B Cooper). If you have already taken the class and need the refresher, it can be done at the same link. Refresher must be done once a year. SINCE CONTRACTS NEED TO BE COMPLETED IN THE FIRST TWO WEEKS OF THE QUARTER, TAKE CARE OF THIS BEFORE ANYTHING ELSE OTHERWISE IT WILL DELAY WORKING IN THE LAB. You will receive an official course “pass” via email at the completion of the course. Please email this to your lab supervisor.
• Must enroll in EH&S “Laboratory Safety Fundamental Concepts Course”, complete the course and obtain final certificate before the SRP contract deadline. (Go to It may take several days to process course completion and receive your certificate. **URC will not accept contracts without this certificate so register for training courses early to avoid delays in your enrollment.**
• In addition to the EH&S safety course, students are highly recommended to complete 2 web-based courses for the UCLA CITI program (Collaborative Institutional Training Initiative). Please goto the appendix under ‘CITI web-based training’ for instructions on completing the free web-based courses.
• Student must print the contract and obtain a signature from Dr. Christopher Cooper (CHS 37-131). Print your SRP contract with your completed Lab Safety Certificate. Give the paperwork to the laboratory coordinator, who will give your contract to Dr. Christopher Cooper’s assistant for signatures. You will be contacted once your contract is ready for pickup in the UC Fit Lab, Room 52-054B in CHS Building.
• Obtain Physiology Department Chair signature. Give your SRP contract (signed by the PI) along with the copy of your Lab Safety Certificate to the administrative assistant in the Physiology Administrative Office, Room 53-263 in CHS Building. You will be contacted once your contract is ready for pickup.
• Student must then take the signed contract to the Registrar’s Office (1st floor of Murphy Hall). Show the desk clerk your contract and student ID and they will add the course to your schedule. This should all be done BY FRIDAY OF THE SECOND WEEK.
• Student completes the experience
• Please note that your first day at the lab may be spent in orientation. The exchange of information should be beneficial to both parties (i.e., student and lab supervisor) in that the student will be given a complete introduction as to the overall aspects of the lab and current research projects. This should include such things as becoming acquainted with department personnel, equipment and facilities available, work schedules, policies & philosophies, administrative procedures, etc. The student in turn will have the opportunity of providing the supervisor with background information relative to ones capabilities and objectives
• During the lab experience, it is up to the student and the supervisor to arrange a working schedule that meets the needs of the facility and one’s own personal and professional needs
• At the end of the student’s experience and by NOON THE FIRST DAY OF FINALS WEEK, students must type (computer-generate) a 1-2 page paper describing their lab experience and hand it in to the lab supervisor. The lab supervisor will then finish filling out the ‘Student EPR Lab Experience Form’ (rate and comment on the students performance and competencies) and sign the form and return it to Dr. Cooper for a signature to submit the student’s grade. Grades will be submitted by the Faulty Mentor at the end of the quarter.